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KMID : 0385520110240030225
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Analytical Science & Technology
2011 Volume.24 No. 3 p.225 ~ p.230
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Analysis of anatoxin-a in aqueous and cyanobacterial samples from korean lakes by liquid chromatography with fluorescence detection
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Lee In-Jung
Lee Chul-Gu
Heo Seong-Nam
Lee Jae-Kwan
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Abstract
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Anatoxin-a is a cyanobacterial neurotoxin with a high toxicity produced by Anabaena, Aphanizomenon and Oscillatoria. Water bloom, formed by Anabaena has been occurring frequently in Lake Yeongchun. It is need to develop a sensitive method for determination of anatoxin-a to control potential hazard in raw water resources. In this study, we developed a highly sensitive analytical method of anatoxin-a using solid phase extraction (SPE) and high performance liquid chromatography (HPLC) with fluorescence detection. Anatoxin-a was converted into a highly fluorescent derivative using 4-fuoro-7-nitro-2,1,3-benzoxadiazole (NBF-F). The method was evaluated in terms of linearity of calibration curve, recovery and repeatability, and the adequate values were obtained. The method detection limit was 0.034 ㎍/g and 0.022 ㎍/ℓ for algal and water samples, respectively. The concentrations of anatoxin-a were measured in algal and water samples from Lake Andong, Yeongchun and Daechung and ranged from 0.135 ㎍/g to 10.979 ㎍/g in algal samples and not detected in water samples.
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KEYWORD
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anatoxin-a, SPE, HPLC, fluorescence detection
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